- DECONTAMINATION OF PESTICIDE RESIDUES ON FRUITS AND VEGETABLES
- By: MD. WASIM AKTAR
The effect of household preparation or commercial processing to reduce the pesticide residue levels on raw agricultural commodities (RACs) is significant as they usually decline the residues. These types of processung strudies are intended: To provide information on the transfer of residues from the RAC to the processed products, in order to calculate reduction factor or concentration factors; To enable and more realistic estimate to be made of the dietary intake of pesticide residues; To establish MRLs for residues in processed products where necessary, according to requirements of national regulatory authorities or international standards. Preferably, RAC samples used in processing studies should contain field treated quantifiable residues as close as possible to the MRL, so that measurable residues are obtained, and transfer factors for the various processed commodities can be determined. A transfer factor gives the ratio of the residue concentration in the processed commodity to that in the RAC. For example if the residue concentration is 0.5 mg/kg in olives and 0.2 mg/kg in olive oil, the transfer factor is 0.2/0.5=0.4. A factor <1 (= reduction factor) indicates and reduction of the residue in the processed commodity, whereas a factor >1 (= concentration factor) indicates a concentration effect of the processing procedures. Enhancing the residues either by increasing the application rates, shortening the pre-harvest interval (PHI) or spiking the RAC with the active ingredient and its metabolites in vitro is not, as and rule, desirable. Spiking is only acceptable if the RAC residues can be shown to consist only of surface residues. However, in some cases, especially where residues in the RAC are close to the analytical limit of determination, field treatment at exaggerated rates or shortened PHIs is advisable to obtain sufficient residue levels for the processing studies.The first step in household or commercial food processing is the preparation of food using various mechanical processes, such as removing damaged or soiled items or parts of crops, washing, peeling, trimming or hulling. This often leads to significant declines in the amount of pesticide residues in the remaining edible portions (Petersen et al., 1996; Celik et al., 1995; Schattenberg et al., 1996).
WASHING
Household washing procedures are normally carried out with running or standing water at moderate temperatures. Detergents, chlorine or ozone can be added to the wash water to improve the effectiveness of the washing procedure (Ong et al., 1996). If necessary, several washing steps can be conducted consequently. The effects depend on the physiochemical properties of the pesticides, such as water solubility, hydrolytic rate constant, volatility and octanol-water partition coefficient (Pow), in conjunction with the actual physical location of the residues; washing processes lead to reduction of hydrophilic residues which are located on the surface of the crops. In addition, the temperature of the washing water and the type of washing has an influence on the residue level. As pointed out by Holland et al. (1994), hot washing and the addition of detergents are more effective than cold water washing. Washing coupled with gentle rubbing by hand under tap water for 1 min dislodges pesticide residues significantly (Barooah and Yein, 1996). Systemic and lipophilic pesticide residues are not removed significantly by washing. Table (1) shows examples of the effects of washing on the residue levels of different pesticides applied to fruits and vegetables.
PEELING
The outer leaves of vegetables often contain residues of pesticides applied during the growing season. Therefore, peeling or trimming procedures reduce the residues levels in leafy vegetables. Peeling of root, tuber and bulb vegetables with a knife is common household practice. Many examples show that most of the residues concentration is located in or on the peel. Peeling of the RACs may remove more than 50% of the pesticide residues present in the commodity. Thus, removal of the peel achieves almost complete removal of residues, so leaving little in the edible portions. This is especially important for fruits which are not eaten with their peels, such as bananas or citrus fruits. Reynolds (1996) showed that peeling or trimming of carrot reduced the residues of chlorfenvinphos, primiphos-methyl, quinalphos, triazophos resulting a transfer factor of 0.2. However, the peel from commercial peeling processes can be used as animal feed or for the production of essential oils (citrus) or pectin (citrus, apple etc.). For such industrial processes, it is important to realize that especially non-systemic surface residues are often concentrated in the peel. For systemic pesticides, peeling may not be as effective as shown by Sheikhorgan et al (1994). After application of thiometon on cucumbers, no reduction of residue levels could be detected in the peeled cucumbers. Under the Codex Alimentarius, as in other international standards, MRLs refer to the whole fruits, which is appropriate for assessing compliance with GAP. These MRLs are of limited significance, however, in assessing dietary exposure to pesticides from fresh fruits, which are peeled (Holland et al.,1994).